Areas of Interest
Research Foci: Chemical biology of hydrogen sulfide signaling, regulation of mammalian sulfur metabolism in health and disease, structural enzymology of human B12 trafficking proteins.
Toxic at high concentrations, H2S is a signaling molecule produced by cells and modulates important physiological processes including blood pressure regulation, inflammation and neuoromodulation. Our laboratory is investigating the reaction mechanisms and regulation of enzymes involved in H2S biogenesis and its clearance via oxidation. In addition to the canonical mitochondrial sulfide oxidation pathway, we have recently discovered a new pathway for clearing H2S, which involves hemeproteins. We use a combination of spectroscopic (EPR, fluorescence), kinetic (stopped-flow spectroscopy) and cellular approaches to understand the mechanisms of catalysis and regulation of key enzymes involved in H2S homeostasis. The enzymes involved in sulfur metabolism are richly dependent on multiple B vitamins for their catalytic functions including vitamin B6, folic acid and B12. My laboratory is also studying the intricate network of chaperones that shepherd and tailor vitamin B12 from its point of entry into cells to its target enzymes and whose dysfunction lead to disease. We have been elucidating novel enzymatic functions of the individual proteins and the thermodynamics and kinetics of protein-protein interactions in the pathway that guide B12 delivery without dilution into the cellular milieu. Using a combination of structural, spectroscopic and kinetic approaches we are studying allosteric regulation in the trafficking pathway for cofactor delivery with high fidelity.
Honors & Awards
Member, American Academy of Arts & Sciences, 2023
Fellow, American Society for Biochemistry and Molecular Biology (ASBMB), 2021
Merck Award, ASBMB, 2019
Associate Editor, Chemical Reviews, 2012–present
Associate Editor, Journal of Biological Chemistry, 2012–present
Fellow, American Association for the Advancement of Science, 2011
Associate Chair, Department of Biological Chemistry, 2008–2019
Pfizer Award in Enzyme Chemistry, American Chemical Society, 2001
Established Investigator, American Heart Association, 2000
Coordination Chemistry Controls Coenzyme B12 Synthesis by Human Adenosine Triphosphate:Cob(I)alamin Adenosyltransferase.
Gouda H, Li Z, Ruetz M, Banerjee R.
Inorg Chem. 2023; 62: 12630–3.
Architecture of the human G-protein-methylmalonyl-CoA mutase nanoassembly for B12 delivery and repair.
Mascarenhas R, Ruetz M, Gouda H, Heitman N, Yaw M, Banerjee R.
Nat Commun. 2023; 14: 4332.
A growth chamber for chronic exposure of mammalian cells to H2S.
Hanna D, Vitvitsky V, Banerjee R.
Anal Biochem. 2023; 673: 115191.
Bivalent molecular mimicry by ADP protects metal redox state and promotes coenzyme B12 repair.
Gouda H, Mascarenhas R, Ruetz M, Yaw M, Banerjee R.
Proc Natl Acad Sci U S A. 2023; 120: e2220677120.
Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer.
Bell HN, Huber AK, Singhal R, Korimerla N, Rebernick RJ, Kumar R, El-Derany MO, Sajjakulnukit P, Das NK, Kerk SA, Solanki S, James JG, Kim D, Zhang L, Chen B, Mehra R, Frankel TL, Győrffy B, Fearon ER, Pasca di Magliano M, Gonzalez FJ, Banerjee R, Wahl DR, Lyssiotis CA, Green M, Shah YM.
Cell Metab. 2023; 35: 134–49.
A redox cycle with complex II prioritizes sulfide quinone oxidoreductase dependent H2S oxidation.
Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R.
J Biol Chem. 2022; 298: 101435.
Gas regulation of complex II reversal via electron shunting to fumarate in the mammalian ETC.
Banerjee R, Kumar R.
Trends Biochem Sci. 2022; 47: 689-98.
Structural perspectives on H2S homeostasis.
Landry AP, Roman J, Banerjee R.
Curr Opin Struct Biol. 2021; 71: 27–35.
Redox-Linked Coordination Chemistry Directs Vitamin B12 Trafficking.
Banerjee R, Gouda H, Pillay S.
Acc Chem Res. 2021; 54: 2003–13.
For a list of publications from PubMed, click HERE